A NOVEL SCANNING SPECTROPHOTOMETER FOR HIGH THROUGHPUT FLUORESCENCE DETECTION

摘要

A fluorescence spectrophotometer having an excitation double monochromator (104), a coaxial excitation/emission light transfer module (106), and an emission double monochromator (110). Each monochromator includes a pair of holographic concave gratings (203, 210) mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well (108) by a light transfer module (106) that includes a coaxial excitation mirror (302) positioned to directed excitation light directly to the bottom of a well (108) of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror (304). The collected emission light is wavelength selected by the emission double monochromator (110). Selected emission light is detected by a photodetector module (112).