PEPTIDES, METHOD FOR ASSAYING HUMAN PEPSINOGEN II or HUMAN PEPSIN II, AND ASSAYING KIT

摘要

Peptide (I) substitutes specific for human pepsin II are new. New peptides and their acid addition salts are of formula (I): [Image] Q : Qa(AA)n; AA : L-amino acid; n : 0-15; QaH, 1-4C alkyl, amino protecting group, D or L-amino acid or NH2(CH2)rCO; r : 2-7; R1, R2H or halo; or R1+R2optionally substituted unsaturated carbocyclyl; R3H or halo; R4H, 1-3C alkyl or hydroxymethyl; EE : D or L amino acid; m : 0 or 1; Q' : aniline, aminocoumarin or aminonaphthalene; provided that R1-R3 are not all H. Independent claims are also included for the following: (1) assaying human pepsin II or human pepsinogen II comprising: (a) using (I) as a substrate and then digesting the peptide of formula (I') which is produced with a peptidase; and (b) measuring the amount of product; and (2) a kit for assaying human pepsin II or human pepsinogen II containing (I): [Image].