Production of proteins, plasmids coding therefor and organisms containing such plasmids

摘要

PCT No. PCT/GB97/00465 Sec. 371 Date Aug. 21, 1998 Sec. 102(e) Date Aug. 21, 1998 PCT Filed Feb. 20, 1997 PCT Pub. No. WO97/31118 PCT Pub. Date Aug. 28, 1997Plasmids which comprise: (1) an origin of replication; (2) an additional sequence required for plasmid replication or preferably a gene giving a selective advantage; and (3) two expression cassettes each of which are located between (1) and (2) but are separated by (1) and (2) from each other; and which are free from inverted repeat sequences (other than in (3)) are highly persistent though successive generations of microbes containing them.