| 摘要 |
PCT No. PCT/US97/18047 Sec. 371 Date Mar. 6, 1999 Sec. 102(e) Date Mar. 6, 1999 PCT Filed Oct. 3, 1997 PCT Pub. No. WO98/15178 PCT Pub. Date Apr. 16, 1998PURPOSE: To produce a novel antibiotic substance zofimarin having antimicrobial activity against fungi and obtain an antimicrobial agent containing the antibiotic substance zofimarin as an active constituent, by cultivating a microorganism, belonging to the genus Zopfiella and capable of producing the zofimarin. CONSTITUTION: An ascomycete belonging to the genus Zopfiella, e.g. Zopfiella marina; is cultivated. The cultivation is carried out according to general fungi, but artifical seawater is used in place of tap water in a liquid culture medium. A carbon source, nitrogen source, inorganic salt, etc., are added, and a defoaming agent is suitably used to carry out the cultivation at 20-30 DEG C. by the spinner culture method with aeration. After completing the cultivation, microbial cells are removed, and the resultant product in the supernatant liquid or filtrate is extracted and purified to afford the aimed zofimarin. The above-mentioned antibiotic substance is neutral colorless oily substance exhibiting the following properties; Soluble in methanol, ethanol and acetone and slightly soluble in water. Positive to the color reaction with iodine and sulfuric acid, etc., and expressed by the formula.
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