发明名称 |
Additives for use in cycling probe reactions |
摘要 |
A method for detecting a target nucleic acid molecule is provided, comprising the steps of (a) reacting a mixture comprising (i) a target nucleic acid molecule; (ii) a single-stranded nucleic acid probe containing a scissile linkage; (iii) an enzyme capable of cleaving the probe portion of a double-stranded target-probe complex at the scissile linkage; and (iv) ribosomal protein and/or spermine, under conditions and for a time sufficient to allow the target nucleic acid and probe to hybridize to each other and form a double-stranded target-probe complex, followed by cleavage of the probe and cycling of the target to a new uncleaved probe, such that one or more portions of the cleaved nucleic acid probe are released from the target-probe complex; and (b) determining whether cleaved portions of the nucleic acid probe are produced, and thereby detecting the presence of the target nucleic acid.
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申请公布号 |
US6136533(A) |
申请公布日期 |
2000.10.24 |
申请号 |
US19980109957 |
申请日期 |
1998.07.02 |
申请人 |
ID BIOMEDICAL |
发明人 |
BEKKAOUI, FAOUZI;MODRUSAN, ZORA D.;PICHE, ISABELLE A.;DUCK, PETER D.;CLONEY, LYNN P.;WONG, ALFRED C. K. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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