Methods of reducing the levels of protoporphyrin IX in recombinant hemoglobin preparations

摘要

PCT No. PCT/US97/18950 Sec. 371 Date Oct. 18, 1999 Sec. 102(e) Date Oct. 18, 1999 PCT Filed Oct. 17, 1997 PCT Pub. No. WO98/17809 PCT Pub. Date Apr. 30, 1998The present invention relates to recombinant hemoglobin solutions containing reduced amounts of protoporphyrin IX and to methods of producing such hemoglobin solutions. The methods are accomplished by overexpressing the hemH gene in a suitable host cell or by increasing the production of hemH in a host cell, for example, by integration of the hemH gene into the chromosome of the host cell or by inserting multiple copies of the gene in host cells naturally having a hemH gene.