| 摘要 |
The present invention pertains to the field of biotechnology, more precisely to that of microbiology, and essentially relates to a method that comprises the following steps: selecting participants for a recombination which are capable of producing mixed microbial communities on a compact nutritive medium; selecting a nutritive medium that can be simultaneously absorbed by all the recombination participants; and preparing a mixture of microbial strains of the recombination participants in a concentration of at least (1-2)x10<4> KOE per ml for each one of them. The method further involves adding from 1 to 5 mu l of a bacteria mixture on the compact nutritive medium, transferring after incubation the communities thus formed from the agar to an isotonic sodium chloride solution and proceeding to the selection. This method is used for producing recombinant micro-organisms which have an improved viability and a higher yield. |
