摘要 |
molecular biology, genetic engineering. SUBSTANCE: invention relates to DNA molecules, recombinant vectors and cellular cultures used for expression of lipase stimulated by bile salts in methyltrophic yeast, Pichia pastoris. DNA molecule has region encoding polypeptide that presents human lipase stimulated by bile salts or its biologically active variant with amino acid sequence given in the invention description. Site is bound to 5'-terminating region encoding signal peptide that is able to operate the secretion of indicated polypeptide from Pichia pastoris cells transformed with indicated DNA molecule with amino acid sequence given in the invention description and methanol oxidizing promoter of Pichia pastoris operative-associated with encoding regions or functionally equivalent promoter. Invention describes plasmid vectors pARC 5771, pARC 5799, pARC 5797. Polypeptide is prepared by culturing host-cells transformed with indicated plasmids and its isolation from cultural medium. EFFECT: increased yield of end product as compared with known methods. 8 cl, 1 tbl, 5 ex
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