| 摘要 |
<p>The present invention relates to a method for determining the presence or absence of a target restriction fragment in a mixture of restriction fragments, said method comprising the steps of: a) contacting the mixture of restriction fragments with the oligonucleotide sequence under hybridizing conditions, such that resulting hybrid has at least one unpaired nucleotide of the target restriction fragment directly adjacent to the 3 end of the oligonucleotide sequence; b) adding at least one labeled nucleotide or nucleotide analog to the mixture of resulting from step a), under conditions suitable for extension of an oligonucleotide; such that the nucleotide sequence is extended with the labeled nucleotide or nucleotide analog; c) detecting the presence or absence of any hybrid with an added labeled nucleotide or nucleotide analog. The oligonucleotide sequences used in step b) are preferably immobilized on a solid support, preferably in the form of an array. The mixture of restriction fragments used preferably is or has been amplified using AFLP.</p> |
