发明名称 |
PRODUCTION OF ATTENUATED CHIMERIC RESPIRATORY SYNCYTIAL VIRUS VACCINES FROM CLONED NUCLEOTIDE SEQUENCES |
摘要 |
Chimeric respiratory syncytial virus (RSV) and vaccine compositions thereof are produced by introducing one or more heterologous gene(s) or gene segment(s) from one RSV subgroup or strain into a recipient RSV background o f a different subgroup or strain. The resulting chimeric RSV virus or subviral particle is infectious and attenuated, preferably by introduction of selecte d mutations specifying attenuated phenotypes into a chimeric genome or antigenome to yield, for example, temperature sensitive (ts) and/or cold adapted (ca) vaccine strains. Alternatively, chimeric RSV and vaccine compositions thereof incorporate other mutations specifying desired structur al and/or phenotypic characteristics in an infectious chimeric RSV. Such chimer ic RSV incorporate desired mutations specified by insertion, deletion, substitution or rearrangement of one or more selected nucleotide sequence(s) , gene(s), or gene segment(s) in a chimeric RSV clone. This provides a method for development of novel vaccines against diverse RSV strains by using a common attenuated backbone as a vector to express protective antigens of heterologous strains. The immune system of an individual is stimulated to induce protection against natural RSV infection, preferably in a multivalent manner to achieve protection against multiple RSV strains and/or subgroups.< /SDOAB>
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申请公布号 |
CA2369200(A1) |
申请公布日期 |
2000.10.19 |
申请号 |
CA20002369200 |
申请日期 |
2000.03.31 |
申请人 |
THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEP ART |
发明人 |
WHITEHEAD, STEPHEN S.;MURPHY, BRIAN R.;COLLINS, PETER L. |
分类号 |
C12N15/09;A61K9/12;A61K39/155;A61P31/14;C07K14/135;C12N7/00;C12N7/04;C12N7/08;C12R1/93;(IPC1-7):C12N15/45 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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