METHOD FOR IDENTIFYING DIFFERENT MAIZE CYTOTYPES AND FOR MEASURING THE CONTAMINATION OF MALE-FERTILE SEEDS MIXED WITH MALE-STERILE SEEDS

摘要

A specific region of the maize mitochondrial genome has been found to have unique sequence in each of four different cytotypes: NA and NB (male fertile), S and C (male sterile). Specific primers allow PCR reactions to amplify, identify and measure each unique sequence. Using the method, said lots can be analyzed for contaminating cytotypes and unidentified cultivars can be typed.