AMPLIFICATION AND SEPARATION OF NUCLEIC ACID SEQUENCES USINGSTRAND DISPLACEMENT AMPLIFICATION AND BIOELECTRONIC MICROCHIP TECHNOLOGY

摘要

Described and disclosed are devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a samp le using novel strand displacement amplification technologies in combination wi th bioelectronic microchip technology. Specifically, a nucleic acid in a sample is amplified to form amplicons, the amplicons are addressed to specified electronically addressable capture sites of the bioelelectronic microchip, t he addressed amplicons are captured and labeled, and then the capture sites are analyzed for the presence of label. Samples may be amplified using strand displacement amplification. The invention is also amenable to other amplification methodologies well known by those skilled in the art. The capture and label steps may be by a method of universal capture with sequenc e specific reporter, or by a method of sequence specific capture with universa l reporter. The label may be detected by fluorescence, chemiluminescence, electrochemiluminescence, or any other technique as are well known by those skilled in the art. This invention further allows for analyzing multiple nucleic acid targets on a single diagnostic platform wherein the nucleic aci ds may be amplified while either in direct contact with microchip components or in solution above themicrochip array.