发明名称 |
Method for treating mammals having a deficiency in endoprotease |
摘要 |
The invention is based on the finding the furin belongs to a family of endoproteolytically active enzymes and relates to a process in the in vitro cleavage of a protein by treating the protein in the presence of a Ca2+ ions with furin, or an endoproteolytically active fragment, derivative or fusion protein of furin. The invention can be used for the (micro) biological production of a protein by culturing genetically engineered cells expressing a pro-form of the protein as well as furin and isolating the protein formed. The invention also relates to a pharmaceutical composition comprising one or more pharmaceutically acceptable carriers, diluents or adjuvants, as well as an endoproteolytically active amount of furin, or a fragment or derivative of furin having an endoproteolytic activity.
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申请公布号 |
US6132717(A) |
申请公布日期 |
2000.10.17 |
申请号 |
US19990253854 |
申请日期 |
1999.02.19 |
申请人 |
KATHOLIEKE UNIVERSITEIT LEUVEN;VAN DE VEN, WILLIAM JAN MARIE |
发明人 |
VAN DE VEN, WILLEM JAN;VAN DEN OUWELAND, ANNA MARIA;VAN DUIJNHOVEN, JOHANNES LAMBERTUS;ROEBROEK, ANTONIUS JOHANNES;KONING, PIET NICO |
分类号 |
A61K38/00;A61K38/48;C07K14/755;C07K14/82;C07K16/32;C07K16/40;C12N9/48;C12N9/62;C12N9/64;C12N15/62;C12P7/64;C12P21/06;(IPC1-7):C12N9/48 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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