发明名称 |
Expression vectors encoding bispecific fusion proteins and methods of producing biologically active bispecific fusion proteins in a mammalian cell |
摘要 |
The present invention provides an expression vector encoding monospecific or bispecific fusion protein. In one embodiment the expression vector encodes a monospecific fusion protein, which vector comprises a recombinant monospecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen. In another embodiment the expression vector encodes a bispecific fusion protein, which vector comprises a recombinant bispecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen and a DNA sequence encoding a second binding domain capable of binding a cell surface antigen, each domain capable of binding a different antigen. The present invention also provides a method for producing a biologically active monospecific or bispecific fusion protein in a mammalian cell. This method comprises: (a) transfecting the mammalian cell with the recombinant expression vector of the invention; (b) culturing the mammalian cell so transfected in step (a); and (c) recovering the biologically active bispecific fusion protein so as produced by the cultured mammalian cell.
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申请公布号 |
US6132992(A) |
申请公布日期 |
2000.10.17 |
申请号 |
US19950539436 |
申请日期 |
1995.10.05 |
申请人 |
BRISTOL-MYERS SQUIBB CO. |
发明人 |
LEDBETTER, JEFFREY A.;GILLILAND, LISA K.;HAYDEN, MARTHA S.;LINSLEY, PETER S.;BAJORATH, JURGEN;FELL, H. PERRY |
分类号 |
C07K14/705;A61K38/00;C07K14/00;C07K14/16;C07K14/715;C07K16/00;C07K16/28;C07K16/30;C07K16/46;C07K19/00;C12N5/10;C12N15/09;C12N15/13;C12N15/62;C12P21/02;C12P21/08;C12R1/91;G01N33/563;G01N33/569;G01N33/574;(IPC1-7):C12P21/02;C12N15/85 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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