METHOD FOR PURIFICATION OF PORCINE TRANSMISSIBLE CORONAVIRUS

摘要

PURPOSE: A method for purifying porcine infectious coronavirus is provided, thereby the porcine infectious coronavirus can be simply and rapidly purified without losing its activity, so that it can be used in producing vaccines or diagnosis kits. CONSTITUTION: The method for purifying porcine infectious coronavirus comprises the steps of: packing the column with crosslinked polysaccharides or sulfate ester of cellulose; washing the column with TEN buffer solution(pH 7.5) consisting of 10mM of Tris, 1mM of EDTA and 100mM of sodium chloride; homogenizing the column with TEN buffer solution; passing the porcine infectious coronavirus containing solution through the column to adsorb it to the column; washing the column with TEN buffer solution; and eluting the porcine infectious coronavirus with TEN buffer solution(pH 7.5) consisting of 10mM of Tris, 1mM of EDTA and 1.0 to 3.0M of sodium chloride.