| 摘要 |
A method has been developed for detection of gene expression or hybridizatio n in microarrays, for example, in combinatorial libraries where quantities are very small and spots located very closely, resulting in uncomfortable situations where intense reaction can spill over into the adjacent spots, an d therefore obscure the accuracy of the reaction of the neighboring sites. The assay uses a digoxigenin enzyme assay for detection. A method for enhancing the reliability of analysis of expression of DNA in microarray formats has also been developed, using software analysis that normalizes the spots. This process uses deformable template techniques to quantify large-scale array da ta automatically, despite possible spatial distortion of the arrays. Each node in the deformable template represents a gene spot, and iterates according to th e gradient descent rule, which minimizes an energy function combining data mismatch energy and template deformation energy.
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