摘要 |
<p>The present invention relates to methods for performing high-throughput biological assays, including immunoassays, nucleic acid detection assays, and related assays. In a preferred embodiment, the target analyte to be detected is a specific mRNA. In another embodiment, the assay monitors the impact of a particular substance or effector on transcription. Alternatively, the target analyte may be a protein, hapten, non-biological chemical, pharmaceutical or other target material. The assay provides high amplification to maximize signal, and utilizes samples which do not require considerable purification.</p> |