SEPARATION OF PHOTOSENSITIZER ISOMERS AND STEREOISOMERS BY LASER-INDUCED FLUORESCENCE CAPILLARY ELECTROPHORESIS

摘要

A method for the separation of isomers and stereoisomers of photosensitizers by Laser-Induced Fluorescence Capillary Electrophoresis has been developed. The limits of detection are 2.06 x 10<-6> M, and the relative standard deviation for the separation was 2.90 % to 4.64 %. Benzoporphyrin derivative mono and diacid (BPD-MA, BPD-DA) enantiomers can be quantitatively determined in the range of 10<-2> to 10<-5> mg mL<-1>. In comparison with HPLC, CE has better resolution and efficiency. This separation method was successfully applied to the BPD enantiomers obtained from a matrix of bovine serum and from liposomally formulated material as well as from studies with rat, dog and human microsomes.