| 摘要 |
The invention relates to a method of determining an analyte in a sample, especially a high concentration analyte, comprises the steps of: a) contacting the sample with a specified amount of a receptor which binds specifically to the analyte to form an analyte/receptor complex, said specified amount of receptor being in excess of that required to bind all analyte in the sample, b) isolating on a solid phase a specified fraction of the amount of receptor contacted with the analyte, including analyte/receptor complex and unreacted receptor, c) detecting the amount of analyte/receptor complex in said isolated specified fraction, and d) from the detected amount analyte/receptor complex, determining the concentration of analyte in the sample. The invention also relates to test kits for carrying out the method. |
