| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new branching enzyme which is derived from Aspergillus nidulans, has a specific amino acid sequence, forms a branch viaα-1,6 bond, and is useful for preventing the aging of starch in food industry and so on. SOLUTION: This is a new branching enzyme which is derived from Aspergillus nidulans, has the amino acid sequence shown by the formula or an amino acid sequence obtained by deleting, substituting, adding, or inserting one or more amino acid(s) from, in, to, or into the amino acid sequence, cleavesα-1,4 bond ofα-glucan such a amylose and amylopectin to form a branch viaα-1,6 bond, and is useful for preventing the aging of starch in food industry and so on. This enzyme is obtained by carrying out PCR of chromosomal DNA of A.nidulans using primers having its partial sequences, screening a genomic library of A.nidulans using the obtained DNA as a probe, followed by expressing the obtained gene in A.nidulans. |
