| 摘要 |
The invention describes novel plane-rigidized cyanine near-infrared (NIR) fluorescent dyes, their methods of preparation and use in the non-isotopic labeling of biological molecules. Such dyes contain a central cyclic hydroanthraquinone framework which provides for a stable and rigid structure to ensure low reactivity with neighboring molecules, a sharp absorption band, a high quantum yield, and emissions in a region largely devoid of natural background auto-fluorescence. Such dyes further comprise variable end groups flanking the central cyclic framework, allowing formation of a number of different plane-rigidized dyes, each having a slightly different emission wavelength. Functional groups are provided for attachment to target biological molecules. Moreover, hydrophilic functional groups are provided where necessary to significantly increase the water solubility of the dye molecules. |
