METHOD FOR SYNTHESIS OF POLYPEPTIDES IN CELL-FREE SYSTEMS

摘要

The method of polypeptide synthesis in eukaryotic or porkaryotic cell-free systems based on a modified version of synthesis in the continuous flow or continuous exchange modes when, in addition to input into the reaction mixtu re of components maintaining the synthesis and removal from the reaction mixtur e of low molecular weight components inhibiting the synthesis, the concentrati on of at least one of the components selected from the group consisting of Mg2+ , K+, NTP, polyamines or their combinations determining the productivity of th e synthesis is continuously changed within the given range of concentrations, while the concentrations of the other components are maintained constant.