High-Throughput-Screening-Verfahren zur Bestimmung der Enantioselektivität asymmetrisch verlaufender Reaktionen

摘要

The present invention relates to a method for determining the enantioselectivity of kinetic racemate resolutions and of asymmetrical reactions of prochiral compounds carrying enantiotopic groups. According to the inventive method, isotopic substrates are used in such a way that an isotope-specific detection system, e.g. an ESI mass spectrometer, can determine the quantity of the reaction products. The method can be combined to an automated sampling and can thus be used for high throughput screening. The enantioselective material conversions relevant in the process can be induced by chiral homogeneous or heterogeneous catalysts, biocatalysts or stoichiometric quantities of optically active agents.