发明名称 |
CELLS COEXPRESSING AN AMYLOID PRECURSOR PROTEIN AND AN alpha -SECRETASE AND THEIR USES IN TEST METHODS AND DIAGNOSTICS |
摘要 |
The invention relates to a recombinant cell which expresses a substrate protein comprising at least one sequence region of 18 amino acids of the human amyloid precursor protein (APP) or a homologous protein. The sequence region contains the alpha -secretase cleavage site as well as 6 amino acid rests at the amino terminal and 12 amino acid rests at the carboxy terminal, in relation to the alpha -secretase cleavage site, and contains either a) a recombinant nucleic acid comprising at least one gene for a protease protein which contains either the sequence region(s), required for proteolytic action, of the disintegrin metalloprotease ADAM 10 obtained from cattle (Bos taurus), humans or another mammal or represents a mutein of a mammalian disintegrin metalloprotease ADAM 10 which has essentially the same enzymatic properties, the gene or genes being controlled by a heterologous promotor; or b) heterologous RNA coding for a substrate protein and a protease protein. The invention also relates to the use of the above cells.
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申请公布号 |
WO0056871(A2) |
申请公布日期 |
2000.09.28 |
申请号 |
WO2000EP02018 |
申请日期 |
2000.03.08 |
申请人 |
FAHRENHOLZ, FALK;POSTINA, ROLF |
发明人 |
FAHRENHOLZ, FALK;POSTINA, ROLF |
分类号 |
A61K38/00;A61K48/00;A61P25/28;C12N9/64;(IPC1-7):C12N15/00;G01N33/53 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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