ELECTROPOLYMERIZABLE FILM, AND METHOD OF MAKING AND USE THEREOF

摘要

An electrode and method of preparing an electrode by electropolymerizing a film on the conductive working surface of an electrode. The electrode is modified by reductive electropolymerization of a thin film of poly[Ru(vbpy)3<2+>] or poly[Ru(vbpy)3<2+>/vba](vbpy = 4-vinyl-4'methyl-2,2'-bipyridine and vba = p-vinylbenzoic acid) and the electrode is used for the electrochemical detection of aqueous GMP, poly[G], and the surface immobilized single-stranded DNA probes. The film is formed from a co-polymer of a mediator such as Ru(vbpy)3<2+> and a functionalized moiety having a carboxylate group such as p-vinylbenzoic acid. A DNA probe is attached covalently to the carboxylate group via a carbodiimide reaction followed by amidation of an amino-linked single-stranded DNA. In the presence of these guanine containing moieties, a dramatic enhancement in the oxidative current for the Ru<3+/2+> couple (present in the polymeric thin film) due to the catalytic oxidation of guanine is observed.