METHOD OF PREPARING CRYOGENICALLY PRESERVED ADHERENT CELL CONTAINING PLATE FOR TISSUE CULTURE APPLICATIONS

摘要

A process for applying and storing adherent cells on a tissue culture plate (20) for preparing tissue culture plates for use in tissue culture experiments. In the process, adherent cells are removed from a flask using trypsin after being incubated in a CO2 incubator. The cell suspension is created using the adherent cells and a liquid culture medium, which is centrifuged and tested for volume using a hemacytometer. The cell suspension is placed in the wells of a tissue culture plate (20) and then incubated. A cryopreservative is deposited into each well and the tissue culture plate is sealed with an adhesive foil cover and a cryogenically preserved using liquid nitrogen. The tissue culture plate (20) is then placed into a plastic, waterproof, resealable bag, enabling it to be stored at -80 DEG C for up to 3 months and shipped without damaging the morphological or functional nature of the adherent cells. The tissue culture plate forms part of a cryopreserved cell culture assembly which includes frozen adherent cells attached to the bottom wall surface of a well in the tissue culture plate.