发明名称 |
METHOD FOR PRODUCING POLYHYDROXYALKANOATES IN RECOMBINANT ORGANISMS |
摘要 |
<p>The present biotechnological approach for the production of polyhydroxyalkanoates (PHAs) uses microbial systems. The high production cos ts make them substantially more expensive than synthetic plastics. Engineering a novel pathway in eucaryotic cell systems is a beneficial alternative to the production of PHAs in bacteria. This pathway will initially produce free (C8 ) fatty acids from the fatty acid synthetic pathway through the action of thioesterase, that will then add a CoA moiety to the free fatty acid through the action of an acyl-CoA synthetase, that will produce 3--ketoacyl-CoAs fro m the acyl-CoA through the action of a thiolase, that will produce R-(-)-OH-ac yl- CoAs from the 3-keto acid CoAs through the action of a dehydrogenase isoform from yeasts. These R-(-)-3-OH-acyl-CoAs will finally be used as substrate fo r the PHA synthase reaction.</p> |
申请公布号 |
CA2365276(A1) |
申请公布日期 |
2000.09.21 |
申请号 |
CA20002365276 |
申请日期 |
2000.03.15 |
申请人 |
UNIVERSITE LAVAL;HER MAJESTY IN RIGHT OF CANADA AS REPRESENTED BY THE MINISTER OF AGRICUL TUR |
发明人 |
VEZINA, LOUIS-P.;AQUIN, STEPHANIE |
分类号 |
C12N9/00;C12N9/04;C12N9/10;C12N9/16;(IPC1-7):C12N15/52;A01H5/00;C12N15/53;C12N15/54;C12N15/55;C12N15/82 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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