| 摘要 |
<p>A method for the modification of a specific gene of interest is described. The method employs two genetic constructs. One construct is a modification plasmid comprising the target gene or nucleic acid fragment of interest. The second construct is a modification cassette comprising yeast selectable markers, and optionally other DNA, bounded by flanking DNA which is complementary to portions of the target gene residing on the modification plasmid. Both constructs are transformed into budding yeast where homologous recombination occurs between the cassette and the plasmid. The resulting altered modification plasmid contains an insertion in the target gene in the regions governed by the sequence of the cassette flanking DNA.</p> |
