摘要 |
<p>PROBLEM TO BE SOLVED: To obtain an environment clarification-related DNA microarray which can efficiently clarify environment polluted air, water, and soil by fixing a specific DNA probe onto a DNA microarray substrate. SOLUTION: This array is obtained by (i) determining a base sequence of at least a part of the genomic DNA or cDNA derived from an environment clarification-related microorganism, (ii) preparing DNA probes based on its base sequence, (iii) fixing the DNA probes onto a DNA microarray substrate, (iv) hybridizing a whole RNA, mRNA, or cDNA derived from the microorganism which was cultured under the condition which allows induction of expression of an environment clarification-related gene with the fixed DNA probes which were obtained in step iii, (v) screening the hybridized DNA probes, followed by (vi) fixing a screened DNA probe onto other DNA microarray substrate. It is preferable to prepare a medium on which data concerning the environment clarification-related microorganism are recorded, and which the data can be read by a computor.</p> |