| 摘要 |
The present invention describes the design and construction of a chimeric insecticidal protein by joining the 5' portion of a synthetic maize optimized cry1B gene (SFLIB) to the 3' end of a full-length synthetic maize optimized cry1A(b) gene to generate a full-length hybrid cry1B gene (hyFLIB). When the chimeric insecticidal protein gene is expressed in transgenic maize from both PEPC and pith promoters, insecticidal activity is observed in transgenic maize tissue against European corn borer (Ostrinia nubilalis). An additional aspect of the invention is recombinant, biologically pure microbial strains transformed with the hyFLIB gene which can be used in entomocidal formulations for the control of Lepidopteran insects. Yet another aspect of the invention is plants transformed with the toxin gene or active fragments thereof, particularly where the transforming sequences have been optimized for expression in maize.
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