Immunoassay utilizing two incubations with labeled antigen

摘要

The present invention relates to an immunoassay for antibodies against an infectious agent. The present invention also relates to the use of the immunoassay and a kit for performing the immunoassay of the present invention. The immunoassay for antibodies against an infectious agent relates to a method comprising: i. incubating a sample suspected of containing said antibodies with immobilized antigen of the infectious agent and free labeled antigen of the infectious agent; ii. separating immobilized components from non-immobilized components; iii. incubating the immobilized components with further free labeled antigen of the infectious agent and removing non-immobilized components; and iv. determining the amount of labeled antigen immobilized, wherein the amount of label is indicative of the amount of said antibodies present in the sample.