| 摘要 |
PURPOSE: A humanized antibody to surface antigen free-S 2 of hepatitis B virus(HBV) is provided, which maintains similar antigen-binding abilities with existing humanized antibody, but diminishes immune-causing abilities than existing humanized antibody obviously, so which can be used to prevent HBV infection and to treat the chronic hepatitis B. CONSTITUTION: A process for the preparation of humanized antibody to HBV surface antigen free-S 2 comprises of: preparing humanized heavy-chained genes; preparing humanized light-chained genes; preparing representing vector(pCMV-HS2(II)HC) producing humanized heavy-chain; preparing representing vector(pKC-dhfr-HS2(II)) producing humanized light-chain; culturing COS7 cells, and washing with OPTI MEM I; mixing pCMV-HS2(II)HC, pKC-dhfr-HS2(II) and Lipofectamine, adding OPTI MEM I, and pouring the mixture over the COS7 cells, culturing, concentrating, and measuring the concentration of the humanized antibody in COS7 cell; investigating binding ability of humanized antibody to HBV surface antigen free-S2; and investigating binding affinity to antigen of humanized antibody.
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