| 摘要 |
PURPOSE: New receptor, kinase CHRK1 and its gene are provided which is used to develop disease resistance plants. CONSTITUTION: PCR primers are synthesized based on conserved amino acid sequences of eukaryote kinases. PCR amplification using RNA isolated from flower as a template is performed and DNA fragment of kinase is obtained. Nicotiana flower cDNA library is screened to isolate full length cDNA of CHRK1 by plaque hybridization method. cDNA in the vector Uni-ZAP XR is transformed into plasmid vector pBluescript by in vivo excision. DNA sequencing reveals that CHRK1 has chitinase like extracellular domain at N-terminal, transmembrane domain and intracellular kinase domain at C-terminal. Genomic DNA analysis of Zea maize, Oryza sativa, Petunia inflata and Brassica oleracea shows that they have CHRK1 homologs. The expression level of CHRK1 is high in flower and very low in root. Virus infected Nicotiana also shows high level of CHRK1 expression. CHRK1 has no chitinase activity but chitin can bind to it.
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