MICROPROPAGATION, SYNTHETIC SEEDS AND GERMPLASM STORAGE OF BAMBOOS

摘要

The invention consists of novel solutions to overcome specific problems currently encountered in propagating bamboo. These solutions may be used individually and in combination with one another. The problems addressed by the invention include the following: a) Specific preparation of plants under stage 0 conditions; b) Use of diseased parts of bamboos as explants for initiation; c) Reversion of in vitro flowering; d) Subculture of flowering parts in vitro as monocultures; d) Induction of nodule cultures in monocultures of flowering parts; f) Induction of flowering in vitro by specific combination of chemical and physical parameter; g) Induction of somatic embryogenesis in adult bamboos without prior organogenesis; h) Production of synthetic seeds; i) Dehydration of somatic embryos prior to the production of synthetic seeds; j) Use of somatic embryos for cryopreservation using an encapsulation dehydration method; k) Use of bottomcooling and increased light intensity prior to transplantation; l) Use of micropropagated plants as micromotherplants for further multiplication; and m) Use of an acetone rinse for sterilization. The invention teaches how to micropropagate bamboo on a large scale with a high percentage success rate.