STABLE RECOMBINANT INFLUENZA VIRUSES FREE OF HELPER VIRUSES

摘要

The invention relates to a recombinant influenza virus for high-yield expression of incorporated foreign gene(s), which is genetically stable in t he absence of any helper virus and which comprises at least one viral RNA segme nt being an ambisense RNA molecule (ambisense RNA segment) and containing one o f the standard viral genes in sense orientation and a foreign, recombinant gen e in anti-sense orientation, or vice versa, in overall convergent arrangement. The present invention further provides a method for the production of said recombinant influenza virus, a method for constructing specific ribozyme- sensitive influenza carrier strains; pharmaceutical compositions comprising said recombinant influenza virus; and the use of said recombinant influenza virus for preparing medicaments for vaccination purposes.