PRODUCTION OF IMMOBILIZED PROTEIN

摘要

PROBLEM TO BE SOLVED: To improve immobilizing yield of a protein in a method for immobilizing the protein through the carboxyl group of carboxy terminal of the protein to a carrier by utilizing amino bond forming reaction through a cyanocysteine residue. SOLUTION: In immobilization of a protein represented by formula I: NH2-R1- COOH (R1 represents arbitrary amino acid residue chain), a protein represented by formula II: NH2-R1-CO-NH-CH(CH2-SCN)-CO-NH-R2-COOH (R1 has the above meaning; R2 represents chain of arbitrary amino acid residue rich in acidic amino acid, strongly negatively charging in nearly neutral and bringing isoelectric point of the compound of formula II to acidity) is reacted with an immobilizing carrier represented by formula III: NH2-Y (Y represents an immobilized carrier having a primary amine as a functional group) to produce the objective immobilized protein represented by formula IV: NH2-R1-CO-NH-Y (R1 and Y have the above meanings).