METHODS FOR LARGE-SCALE PRODUCTION OF RECOMBINANT AAV VECTORS
摘要
Disclosed are HSV-1 amplicons that supply all necessary helper functions required for rAAV packaging and methods for their use. These HSV-1 amplicons have been shown to be capable of rescuing and replicating all forms of rAAV genomes including rAAV genomes introduced into cells by infection of rAAV virions, rAAV genomes transfected into cells on plasmids or proviral rAAV genomes integrated into cellular chromosomal DNA. Also provided are methods for preparing high-titer rAAV vector compositions suitable for gene therapy and the delivery of exogenous polynucleotides to selected host cells.
申请公布号
WO0017377(A3)
申请公布日期
2000.09.08
申请号
WO1999US22052
申请日期
1999.09.22
申请人
UNIVERSITY OF FLORIDA;THE JOHNS HOPKINS UNIVERSITY;BYRNE, BARRY, J.;CONWAY, JAMES, E.;HAYWARD, GARY, S.;MUZYCZKA, NICHOLAS;ZOLOTUKHIN, SERGEI
发明人
BYRNE, BARRY, J.;CONWAY, JAMES, E.;HAYWARD, GARY, S.;MUZYCZKA, NICHOLAS;ZOLOTUKHIN, SERGEI