CRYSTALS AND STRUCTURAL COORDINATE OF PROTEIN COMPLEX AND UTILISATION OF THE STRUCTURAL COORDINATE

摘要

Crystals of a protein complex composed of granulocyte colony-stimulating factor (G-CSF) and the G-CSF binding moiety of a G-CSF receptor; and the structural coordinate of each atom determined by the crystal structure analysis procedure from these crystals. By using this structural coordinate, it is possible to identify, search, evaluate or design a G-CSF mutant which has a biological activity higher than G-CSF occurring in nature or an inhibitory activity on G-CSF and is derived by substitution, deletion, insertion or chemical modification of one or more amino acid residues, an agonist which is a compound having a biological activity comparable or superior to the biological activity of G-CSF, and an antagonist which is a compound inhibiting the biological activity of G-CSF.