| 摘要 |
<p>The present invention relates to DNA-based constructs and methods for treating viral infections. In a preferred embodiment, an HSV based amplicon is topically applied to the site of an actively replicating HSV infection, initiating a four-tier anti-viral therapy. First, IFN-η expression from a constitutive promoter exerts an antiviral effect at the site of active infection. Second, replication machineray of the resident virus packages the amplicon into HSV virions, in situ, thereby decreasing the infectious titer. Third, HSV virion containing amplicon DNA are transported into the nervous system where IFN-η expression promotes the establishment of latency. Fourth, as the host is exposed to emotional or physical stressor that reactivate the virus, amplicon-directed IFN-η is expressed. Increasing titers of IFN-η enforce viral latency.</p> |
