METHOD TO DETECT ANTIMICROBIAL AGENT RESISTANT MICROBES

摘要

A method to detect in a sample the presence or absence of microbial cells that are resistant to at least one antimicrobial compound, the method comprising a selective enrichment step comprising the substeps of subjecting the sample to a first enrichment step in a medium and under conditions that will permit selective enrichment of both resistant and non-resistant cells of the pre-selected species or groups of species, followed by the substep of subjecting the thus enriched sample to an enrichment step in a medium and under conditions that will permit selective enrichment of resistant cells of the pre-selected species or group of species, and a step of detecting the presence of absence or resistant cells. The method permits the absence/presence of resistant organisms such as e.g. Salmonella to be detected in about 24 hours.