Hemoglobin mutants that reduce heme loss

摘要

PCT No. PCT/US96/16934 Sec. 371 Date Apr. 22, 1998 Sec. 102(e) Date Apr. 22, 1998 PCT Filed Oct. 23, 1996 PCT Pub. No. WO97/15591 PCT Pub. Date May 1, 1997The present invention relates to methods of reducing heme loss in hemoglobins to produce stability and improve expression yield of hemoglobins, particularly recombinant hemoglobins. Such methods are accomplished by introducing mutations in the alpha or beta subunits of hemoglobins to increase heme affinity. The present invention further relates to novel mutations that reduce such heme loss.