发明名称 |
Oligonucleotide modification, signal amplification, and nucleic acid detection by target-catalyzed product formation |
摘要 |
A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5'-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5'-nuclease to cleave the oligonucleotide to provide (i) a first fragment that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3' of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte. The method has particular application to the detection of a polynucleotide analyte such as DNA. Kits for conducting methods in accordance with the present invention are also disclosed.
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申请公布号 |
US6110677(A) |
申请公布日期 |
2000.08.29 |
申请号 |
US19980015949 |
申请日期 |
1998.01.30 |
申请人 |
DADE BEHRING MARBURG GMBH |
发明人 |
WESTERN, LINDA M.;ROSE, SAMUEL J.;ULLMAN, EDWIN F. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12Q1/48;C12Q1/44;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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