| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for promptly and accurately discriminating the number and kind of both living and dead fungi by effectively making a fluorescent dye penetrate and stain the inside of cells of various living and dead fungi and by irradiating liquid containing the fluorescent dye and the fungi in which fluorescent dye particles not penetrating the cells are prevented from firefly luminescence by intracellular enzyme with pulsed excitation light having high light energy and proper excitation wavelength. SOLUTION: This method for promptly discriminating the number and kind of both lining and dead fungi is to irradiate liquid 4 containing a fluorescent dye and fungi with pulsed excitation light 6 having high light energy and proper excitation wavelength to cause high-intensity firefly luminescence. In the liquid 4 the inside of living fungus cells is effectively penetrated and stained with a fluorescent dye for staining living fungus cells such as acetoxymethyl esterified calcein or acetoxymethyl esterified BCECF, or CFSE, carboxylfluorescein diacetate or fluorescein diacetate together with a dyeing accelerator comprising salts, chitinase and cellulase, and the inside of dead fungus cells is penetrated and stained with a fluorescent dye for staining dead fungus cells such as propidium iodide, ethidium bromide or ethidium homodimer.
|
