| 摘要 |
Production of recombinant adenovirus (A) comprises: (a) introducing viral DNA into a culture of packaging cells, and (b) harvesting (A) produced after their release into the supernatant. Also claimed are: (1) purification of (A) from a medium by chromatography on a strong anion exchanger; (2) (A) prepared as described above; (3) use of iodixanol (I; 5,5'-[(2-hydroxypropane-1,3-diyl)bis(acetylamino)] bis[N,N'-bis(2,3-dihydroxypropyl) -2,4,6-triiodobenzene-1,3-dicarboxamide) for purification of adenovirus, and (4) purification of adenovirus from medium by: (i) ultracentrifugation; (ii) dilution or dialysis, and (iii) anion-exchange chromatography. |
