| 摘要 |
PURPOSE: Provided is a method of preparing D-aspartic acid and β -alanine by enzymatic decarboxylation of racemic aspartic acid. CONSTITUTION: A racemic mixture of aspartic acid isomers (D and L) are incubated with a microorganism containing the panD gene encoding the enzyme L-aspartate-α -decarboxylase (E.C. 4.1.1.11) or a suitable source of enzyme. The enzyme catalyzes the following reaction. The enzyme stereospecifically decarboxylates L-aspartate to β -alanine and CO2 whereas D-aspartate remains unreacted. Because this reaction is essentially irreversible with the release of CO2, a high conversion of product is realized. This reaction can be carried out under mild conditions, i.e., room temperature and neutral pH, thus making this process very environmentally friendly and highly desirable.
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