| 摘要 |
PURPOSE: Recombinant organisms are provided comprising genes encoding glycerol-3-phosphate dehydrogenase, glycerol-3-phosphatase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates. CONSTITUTION: A method for the production of 1 ,3-propanediol from a recombinant organism comprises: (i) transforming a suitable host organism with a transformation cassette comprising at least one of (a) a gene encoding a glycerol-3-phosphate dehydrogenase activity; (b) a gene encoding a glycerol-3 phosphatase activity; (c) genes encoding a dehydratase activity; and (d) a gene encoding 1 ,3-propanediol oxidoreductase activity, provided that if the transformation cassette comprises less than all the genes of (a)-(d), then the suitable host organism comprises endogenous genes whereby the resulting transformed host organism comprises at least one of each of genes (a)-(d); (ii) culturing the transformed host organism under suitable conditions in the presence of at least one carbon source selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, or a one carbon substrate whereby 1,3-propanediol is produced; and (iii) recovering the 1,3-propanediol. |
