发明名称 |
Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells |
摘要 |
The present invention concerns a method of preparing a conditioned medium from a confluent stromal cell culture, the method comprising:
(a) culturing stromal cells to confluence in a stationary phase plug-flow bioreactor under continuous flow of medium on a three dimensional substrate to obtain a stationary phase three-dimensional confluent stromal cell culture, wherein said stromal cell culture is grown to a density of at least 5 x 10 6 cells per a cubic centimeter of said substrate; and
(b) separating and collecting a stromal cell conditioned medium from said stationary phase confluent stromal cell culture; thereby obtaining the conditioned medium. |
申请公布号 |
AU3480700(A) |
申请公布日期 |
2000.08.25 |
申请号 |
AU20000034807 |
申请日期 |
2000.02.04 |
申请人 |
TECHNION RESEARCH & DEVELOPMENT FOUNDATION LTD. |
发明人 |
SHAI MERETSKI |
分类号 |
C12M3/00;A61F2/00;A61K35/12;A61K35/14;A61P7/00;A61P43/00;C12M3/04;C12N5/00;C12N5/02;C12N5/078;C12N11/08;C12R1/91 |
主分类号 |
C12M3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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