| 摘要 |
A method of comparing genomic DNA from two individuals who share a phenotype, cutting the DNA into fragments long enough to contain on average one or several polymorphisms; combining the fragments under hybridisation conditions and recovering mismatch-free heterohybrids, wherein adapters resistant to nuclease digestion are ligated to the ends of the genomic fragments. A related method uses pooled genomic DNA from individuals with a common phenotype. Another related method uses restricted nucleic acid fragments likely to contain on average less than one natural polymorphism. |
