发明名称 |
Method for conveying BNYVV resistance to sugar beet plants |
摘要 |
<p>The present invention relates to a method for conveying resistance to beet necrotic yellow vein virus (BNYVV) to a sugar beet plant, which method comprises the following steps: (a) preparing a DNA fragment of at least 15 nucleotides in a sequence that is essentially homologous to the corresponding nucleotide sequence of the genomic RNA 1 of the beet necrotic yellow vein virus (BNYVV), (b) introducing said DNA fragment, operately linked to a promotor that is active in sugar beet plants, into a sugar beet plant cell to obtain a transformed sugar beet cell; and (c) regenerating a transgenic sugar beet plant from the transformed sugar beet plant cell. In addition, the invention relates to a DNA vector harboring a fragment of at least 15 nucleotides in a sequence that is essentially homologous to the corresponding nucleotide sequence of the genomic RNA 1 of BNYVV and transcription and translation regulatory sequences operably linked therewith, as well as to the use of said DNA vector for the transformation of a plant cell. The invention further relates to a plant cell comprising in its genome a DNA fragment of at least 15 nucleotides in a sequence that is essentially homologous to the corresponding nucleotide sequence of the genomic RNA 1 of BNYVV and the use of said plant cell for the regeneration therefrom of a sugar beet plant that is resistant against BNYVV. <IMAGE></p> |
申请公布号 |
EP1029923(A1) |
申请公布日期 |
2000.08.23 |
申请号 |
EP19990200236 |
申请日期 |
1999.01.27 |
申请人 |
D.J. VAN DER HAVE B.V. |
发明人 |
RICHARDS, KENNETH;JONARD, GERARD;GUILLEY, HUBERT;VAN DUN, CORNELIS MARIA PETRUS |
分类号 |
A01H5/00;C07K14/08;C12N5/10;C12N15/40;C12N15/82;(IPC1-7):C12N15/82 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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