Non-optimal Kozaks sequences

摘要

A non-optimal translational start site (Kozak) polynuclcotide sequence as set forth in SEQ ID NO:2 is provided. An expression vector for expressing a protein of interest, utilizing the non-optimal translational start site is also provided. Host cells containing the expression vector utilizing the non-optimal translational start site are also provided. A selectable marker encoded by a nucleic acid sequences utilizing the non-optimal translational start site is further provided.